The Effect of Delivery Matrix on Bifidobacterium animalis subsp. lactis HN019 Survival through In Vitro Human Digestion

Bifidobacterium animalis subsp. lactis HN019 is a probiotic with several documented human health benefits. Interest in probiotics has led to the development of new formats that probiotics, including HN019, can be supplemented into. In this study, we looked at common such as frozen culture and freeze-dried powder well supplementing it into following food matrices: yogurts (dairy, soy, oat based), xanthan gum-based tablets, pulpless orange juice, whey sports drink, dark chocolate (70% cocoa). work, our aim was investigate whether matrix carried via simulated digestion (a dual model system mimicking both upper lower gastrointestinal digestion) influenced delivery. To end, validated used real-time qPCR assay detect after digestion. addition, also measured effect on panel metabolites. After digestion, were able from all matrices tested, observed changes metabolite profile consistent those expected used. conclusion, work suggests did not interfere delivery colon